Rotavirus Rapid Test Kit

Short Description:

A rapid, one step test for the qualitative detection of rotavirus in human feces.

MOQ:1000 pcs


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SUMMARY

Rotavirus is the most common agent responsible for acute gastroenteritis, mainly in young children.1 Its discovery in 1973 and its association with infantile gastro-enteritis represented a very important advancement in the study of gastro-enteritis not caused by acute bacterial infection. Rotavirus is transmitted by oro-faecal route with an incubation period of 1-3 days. Although specimen collections taken within the second and fifth day of the illness are ideal for antigen detection, the rotavirus may still be found while diarrhoea continues. Rotaviral gastroenteritis may result in mortality for populations at risk such as infants, the elderly, and immunocompromised patients.2 In temperate climates, rotavirus infections occur mainly in the winter months. Endemics as well as epidemics affecting some thousand people have been reported.3 With hospitalised children suffering from acute entric disease up to 50% of the analysed specimen were positive for rotavirus.4 The viruses replicate in the cell nucleus and tend to be host species specific producing a characteristic cytopathic effect (CPE). Because rotavirus is extremely difficult to culture, it is unusual to use isolation of the virus in diagnosing an infection. Instead, a variety of techniques have been developed to detect rotavirus in feces. The Rotavirus Rapid Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of rotavirus in human feces specimen, providing results in 10 minutes. The test utilizes antibody specific for rotavirus to selectively detect rotavirus from human feces specimens.

PRINCIPLE

The Rotavirus Rapid Test Cassette (Feces) is a qualitative, lateral flow immunoassay for the detection of rotavirus in human feces specimens. In this test, the membrane is precoated with anti-rotavirus antibody on the test line region of the test. During testing, the specimen reacts with the particle coated with anti-rotavirus antibody. The mixture migrates upward on the membrane chromatographically by capillary action to react with anti-rotavirus antibody on the membrane and generate a colored line in the test line region. The presence of this colored line in the test region indicates a positive result, while its absence indicates a negative result. To serve as a procedural control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

STORAGE AND STABILITY

The kit can be stored at room temperature or refrigerated (2-30°C). The test cassette is stable through the expiration date printed on the sealed pouch. The test cassette must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.

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