Glucose-6-Phosphate Dehydrogenase (G6PD) is a critical enzyme in the pentose phosphate pathway (PPP), a metabolic pathway that plays a key role in red blood cell (RBC) metabolism.

G6PD deficiency is a hereditary enzyme disorder caused by a defect in the G6PD enzyme of red blood cell membranes. This defect leads to a decrease in the production of reduced nicotinamide adenine dinucleotide phosphate (NADPH), which is essential for maintaining the stability of red blood cell membranes and protecting against oxidative damage. The deficiency can result in red blood cell destruction and hemolysis. Patients with G6PD deficiency are unable to metabolize glucose properly, with exposure to oxidative substances such as fava beans, aspirin and sulfonamide drugs can trigger acute hemolytic reactions, leading to symptoms such as jaundice, mental impairment, and, in severe cases, rapid breathing heart failure, and shock, which can be life-threatening. Therefore, the measurement of G6PD enzyme activity is of great clinical significance.

The One Step G6PD Blood Test Kit is used for qualitative detection of normal or deficient G6PD enzyme activity in whole blood samples utilizing the glucose-6-phosphate substrate colorimetric method. In the presence of normal G6PD enzyme activity, insoluble blue-purple crystals are formed. The test kit includes a filtration membrane that removes hemoglobin from the sample, allowing the purple crystals to bind to the membrane and form a visible blue-purple circular spot. However, when G6PD enzyme activity is defect, the blue-purple circular spot cannot be formed, indicating a deficiency in G6PD enzyme.

The G6PD Test kit is easy to operate and can be conveniently used in various medical settings, using only 10 microliters of whole blood sample (fingertip blood, earlobe blood, venous blood applicable) for testing. The immediate test result can be obtained visually through simply color comparison in a very short time with purple, blue-purple or black color indicating a normal G6PD enzyme activity, and pink, yellow or white color indicating a deficiency in G6PD enzyme activity.

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