Typhoid Antigen Rapid Test

Short Description:

The Typhoid Ag Rapid Test Device (Feces) is a lateral flow immunoassay for the simultaneous detection and differentiation of Salmonella Typhoid in feces.

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SUMMARY

Typhoid fever is caused by S. typhi, a Gram-negative bacterium. World-wide an estimated 17million cases and 600,000 associated deaths occur annually1. Patients who are infected withHIV are at significantly increased risk of clinical infection with Typhoid². Evidence of H.pyloriinfection also presents an increase risk of acquiring typhoid fever. 1-5% of patients becomechronic carrier harboring S. typhi or S. Para typhi in the gallbladder.The clinical diagnosis of typhoid fever depends on the isolation of Typhoid from blood, bonemarrow, feces or a specific anatomic lesion. In the facilities that can not afford to perform thiscomplicated and time-consuming procedure, Filix-Widal test is used to facilitate the diagnosis.However, many limitations lead to difficulties in the interpretation of the Widal test3,4.In contrast, the Typhoid Ag Rapid Test Device (Feces) is a simple and rapid laboratory test. Thetest simultaneously detects S. typhoid antigen thus to aid in the determination to the S.typhiod.

PRINCIPLE

The Typhoid Ag Rapid Test Device (Feces) is a lateral flow chromatographic immunoassay. Thetest cassette consists of: 1) a burgundy colored conjugate pad containing S. typhoid antibodyconjugated with colloid gold, 2) a nitrocellulose membrane strip containing two test bands (S.typhoid bands) and a control band (C band). The S. typhoid band is pre-coated withmonoclonal anti- S. typhoid for the detection of S. typhoid Ag, and the C band is pre-coatedwith goat anti mouse IgG.When an adequate volume of test specimen is dispensed into the sample well of the cassette,the test specimen migrates by capillary action across the test cassette. S. typhoid Ag if presentin the patient specimen will bind to the S. typhoid Ab conjugates. The immunocomplex is thencaptured on the membrane by the pre-coated S. typhoid antibody, forming a burgundy coloredS. typhoid band, indicating a S. typhoid positive test result.Absence of any test bands suggests a negative result. The test contains an internal control (Cband) which should exhibit a burgundy colored band of the immunocomplex of goat antiMouse IgG/Mouse IgG-gold conjugate regardless of the color development on any of the testbands. Otherwise, the test result is invalid and the specimen must be retested with anotherdevice.

STORAGE AND STABILITY

Store as packaged in the sealed pouch at room temperature or refrigerated (2-30℃). The test is stable through the expiration date printed on the sealed pouch. The test must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.

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