TOXO IgG Rapid Test Kit

Short Description:

A rapid test for the qualitative detection of IgG antibodies to Toxoplasma Gondii(T.gondii) in human serum or plasma

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【SUMMARY】

T. gondii is an obligate intracellular protozoan parasite with a worldwide distribution1,2. Serological data indicates that approximately 30% of the population of most industrialized nations is chronically infected with the organism3. A variety of serologic tests for antibodies to T. gondii have been used as an aid in diagnosis of acute infection and to assess previous exposure to the organism. These tests are the SabinFeldman dye test, direct agglutination, indirect hemagglutination, latex agglutination, indirect immunofluorescence, and ELISA. Recently, lateral flow chromatographic immunoassay, such as The Toxo IgG Rapid Test Cassette was introduced into the clinic for the serodiagnosis of T. gondii infection.

【PRINCIPLE】

The Toxo IgG Rapid Test Cassette is a lateral flow chromatographic immunoassay. The test consists of: 1) a colored conjugate containing recombinant T. gondii antigens conjugated with colloid gold (T. gondii conjugates) and mouse IgG-gold conjugates, 2) a nitrocellulose membrane strip containing a test band (T band) and a control band (C band). The T band is pre-coated with monoclonal anti-human IgG for detection of IgGanti-T. gondii , and the C band is pre-coated with goat anti mouse IgG.When an adequate volume of test specimen is applied into the sample pad of the test, the specimen migrates by capillary action across the strip. IgG anti-T. gondii if present in the specimen will bind to the T. gondii conjugates. The immunocomplex is then captured on the membrane by the pre-coated monoclonal anti-human IgG, forming a colored T band, indicating a T. gondii IgG positive test result.Absence of any T band (T) suggests a negative result. The test contains an internal control (C band) which should exhibit a colored band of the immunocomplex of goat anti mouse IgG/mouse IgG-gold conjugate regardless of the color development on the T band. Otherwise, the test result is invalid and the specimen must be retested with another device.

【STORAGE AND STABILITY】

Store as packaged in the sealed pouch at room temperature or refrigerated (2-30℃). The test is stable through the expiration date printed on the sealed pouch. The test must remain in the sealed pouch until use. DO NOT FREEZE. Do not use beyond the expiration date.

Toxo rapid test kit Toxo test kit

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