PCR Test Principle

Polymerase Chain Reaction (PCR) is an in vitro nucleic acid amplification technique developed in the mid-1980s. It can expand the target gene or a DNA fragment to one hundred thousand or even one million times in a few hours in a test tube, so that the naked eye can directly observe and judge; Enough DNA can be amplified from a hair, a drop of blood, or even a cell for analysis and identification. PCR testing is a revolutionary initiative and milestone in the field of biomedicine. In fact, the PCR laboratory is extremely powerful, qualitative analysis and quantitative detection are its two biggest application directions, then in addition to the novel coronavirus nucleic acid detection, what else can our "universal" PCR laboratory do? Next, I will show you some examples of projects in specific application directions.

 

Pathogen assay

The advent of PCR technology enables pathogen detection to be carried out conveniently, with strong specificity, high sensitivity, simple operation, time-saving and other characteristics, it can not only be used for basic research such as gene isolation and cloning and nucleic acid sequence analysis, but also for disease diagnosis and any place where there is DNA and RNA. Due to the high false positive rate of PCR technology, positive results can be obtained as long as there is a trace of pathogens, which cannot be used as a diagnostic basis, and only when a certain number of pathogens exist, it has clinical significance. Therefore, the accurate quantification of the template is particularly important, and the results can be obtained quickly and accurately by using fluorescence PCR. To solve the "window period" problem of immunological detection, to determine whether the disease is in a recessive or subclinical state, and to determine whether the antibody test can not determine the current infection or previous infection, PCR can be used to determine. It is used for in vitro qualitative detection of suspected cases of pneumonia infected by the novel coronavirus, suspected clusters of patients, and other cases requiring the diagnosis or differentiation of novel coronavirus infection.

Genetic disease detection

Gene mutation and copy number variation are the main genetic basis of genetic diseases and the main targets of genetic disease detection. The complexity of genetic diseases is accompanied by the large number and wide types of gene mutations. Gene copy number variation is not only represented by deletion and replication, but also by deletion location, size and replication multiple. The complexity of genetic variation poses a technical challenge for the clinical detection of genetic diseases. Real-time PCR technology is a new generation of real-time PCR technology that we have recently developed, using fluorescent labeling or melting point analysis, can detect multiple targets in a single reaction tube.

Oncology test

Tumor is a kind of disease that endangers human health. Its malignant degree and prognosis mainly depend on whether there is metastasis and pathological section stage. With the rapid development of molecular biology, a large number of research results in the field of tumor research show that the occurrence, development, treatment and prognosis of tumors are related to the genes in tumor cells and tumor-related viruses. Detection of the expression of many proto-oncogenes and tumor suppressor genes can reveal the possibility of carcinogenesis, and PCR methodology can be used for oncology genetic testing. In addition, PCR method is used for qualitative detection of septin9 gene methylation in human peripheral blood plasma in vitro, and is used for clinical auxiliary diagnosis of various tumors.

 

Using the pcr test antigen test of HYSEN can quickly detect the results!


Post time:2024-01-25
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