Human monkeypox (HMPX), caused by the monkeypox virus (MPXV) which is a double stranded DNA virus, a member of the orthopoxvirus genus within the Poxviridae family. It is a viral zoonotic disease, meaning that it can spread from animals to humans. It can also spread between people.The incubation period of monkeypox can range from 5 to 21 days. The febrile stage of illness usually lasts 1 to 3 days with symptoms including fever, intense headache, lymphadenopathy (swelling of the lymph nodes), back pain, myalgia (muscle ache), and an intense asthenia (lack of energy). The febrile stage is followed by the skin eruption stage, lasting for 2 to 4 weeks. Lesions evolve from macules (lesions with a flat base) to papules (raised firm painful lesions) to vesicles (filled with clear fluid) to pustules (filled with pus), followed by scabs or crusts.For Monkeypox virus detection, Hysen can offer two different methods based one different technology.

  The first one is Human Monkeypox Virus Test Cassette. The Human Monkeypox Virus Test Cassette is a qualitative membrane-based immunoassay for the detection of human monkeypox virus antigen in lesion exudate/ oropharyngeal swab. In this test, antibody specific of Monkeypox Virus is separately coated on the test line regions of the test cassette. During testing, the extracted specimen reacts with the antibody of Monkeypox Virus that are coated onto particles. The mixture migrates up the membrane to react with the antibody of Monkeypox Virus on the membrane and generate one colored line in the test regions. The whole procedure only takes about 10 minutes to get the result. The presence of this colored line of the test regions indicates a positive result. To serve as a procedural control, a colored line will always change from Blue to Red in

the control line region, indicating that membrane wicking has occurred.

  If you need more sensitive test result, we would suggest the other test method. The other one is Monkeypox Virus Nucleic Acid Test Card with relevant analyzer. The assay is based on isothermal amplification method and enzymedigestionprobe technology, and conserved regions are selected for specific primers and specific probes design. A large number of target sequence's copies weregenerated in the reaction system during the isothermal amplification. When the probe hybridizes to the complementary sequence, it is cleaved and fluorescence is emitted. Integrated Nucleic Acid Testing Device detects and analyzes fluorescence signal automatically, reporting negative, positive orinvalid result. The assay includes internal control for monitoring of sample collection, processing, and amplification to reduce false negative results.

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Post time:2024-08-22