Dengue Antigen/Antibody Combo Rapid Test Kit

  Dengue is a flavivirus, transmitted by Aedes aegypti and Aedes albopictus mosquitoes. It is widely distributed throughout the tropical and subtropical areas of the world,mostly in urban and semi-urban areas.Dengue causes up to 100 million infections annually.It is the most severe arbovirus disease that causes human morbidity and death.Today,about 2.5billion people live in areas where there is a risk of dengue transmission.Clinic feature of Dengue infection are sudden onset of fever, intense headache, myalgia, arthralgia and rash. Primary Dengue infection causes IgM antibodies to increase to a detectable level in 3 to 5 days after the onset of fever. IgM antibodies generally persist for 30 to 90 days.Most Dengue patients in endemic regions have secondary infections, resulting in high levels of specific IgG antibodies prior to or simultaneous with IgM response. Therefore, the detection of specific anti-Dengue IgM and IgG antibodies can also help to distinguish between primary and secondary infections.NS1 is one of seven Dengue Virus non-structural proteins which are thought to be involved in viral replication.NS1 exists as a monomer in its immature form but is rapidly processed in the endoplasmic reticulum to form a stable dimer. It allows rapid detection on the first day of fever, before antibodies appear some 5 or more days later.A small amount of NS1 remains associated with intracellular organelles where it is thought to be involved in viral replication. The rest of NS1 is found either associated with the plasma membrane or secreted as a soluble hexadimer. NS1 is essential for viral viability but its precise biological function is unknown.Antibodies raised in response to NS1 in viral infection can cross react with cell surface antigens on epithelial cells and platelets and this has been implicated in the development of Dengue Hemorrhagic fever.Early detection and access to proper medical care can lower fatality below 1%. 

  The Dengue Ab/Ag Combo Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid test for the qualitative detection of antibodies (IgG and IgM) and NS1 antigen to Dengue virus in whole blood, serum, or plasma.It has been evaluated with specimens obtained from a population of symptomatic and asymptomatic individuals. Results were confirmed by a leading commercial Dengue ELISA test.The results show that the overall relative sensitivity for the primary and secondary infection of the Dengue Abs Rapid Test Cassette (Whole Blood/Serum/Plasma) is95.7% , and the relative specificity is >99.9%,and the relative accuracy is 99.3%, and for the Dengue NS1 Ag Rapid Test Cassette (Whole Blood/Serum/Plasma) is 95.8%,and the relative specificity is 96.2%, and the accuracy is 96.1%.